This research investigated and selected 4 natural populations of Picea likiangensis in Yunnan province, based on its natural distribution. The length, width, dry weight and C, N, P contents in needles of the 4 populations were measured to survey the phenotypic variation of natural populations and the relationship between the phenotypic variation and their altitude of distribution areas. The methods of ANOVA analysis, multi-comparison and correlation analysis were used for analyzing the data measured. The results showed that, there were remarkable (PPPPPPP<0.05) positively correlated.